![]() LFO also had to fight against the conventional wisdom of the time, which held that any young male pop group was by definition a boy band, whether they had advanced choreography like N’Sync or were a traditional guitar-playing pop-rock group like BBMak. “I knew he didn’t really care for us.” Fischetti won’t name names, but here’s the video for their second single “Girl on TV,” starring Jennifer Love Hewitt. “There was some support, but there were some people there who didn’t think we were interesting or important.” He recalls popping his head into a certain VJ’s dressing room to say hello before an appearance and getting the cold shoulder. “We had an interesting relationship with MTV,” Fischetti says, carefully. LFO had a hit single, but maybe not the respect that comes with it, even on TRL. “Summer Girls” went to number three in the summer of 1999, right between Christina Aguilera’s “Genie in a Bottle” and Smash Mouth’s “All Star” and if you have forgotten how packed that summer was, consider this: Backstreet Boys’ “I Want It That Way” only made it to number six. I say: it may not make sense, but it did make dollars.” “I can tell you Rich was not on acid or anything like that,” he hastens to point out. ![]() ![]() (Even the users of can’t help you all they have to say about this line is that “marmalade is jam-like breakfast spread, often put on toast.”) The lyrics are so stream-of-consciousness, I tell Fischetti, that they almost qualify as psychedelia. The first verse starts “Hip-hop, marmalade, Spic-and-Span,” and things get weirder from there. “And it allowed us to show that we could write music, which most groups like us didn’t do at the time.” “Summer Girls” is breezy and infectious, and-I say this with awe and respect-one of the strangest pop hits of all time. LFO went platinum, largely off the popularity of the pop rap single “Summer Girls.” “We really did start as a rap group, so hitting with a sing-songy rap song was really important,” Fischetti says. Their self-titled debut album came out right at the perfect moment, just as Backstreet Boys, Britney Spears, and N’Sync made pop music cool again, right when TRL was taking off. An open audition brought Lima into the fold, he suggested shortening the name, and LFO was born. Brizz got antsy and split for a solo career. New Kids on the Block had had a bunch of hits, but the mid-‘90s mood was a little darker, a little less poppy. ![]() In 1995, when Fischetti and Cronin formed Lyte Funkie Ones with a singer named Brian “Brizz” Gillis, teenage girls were not snapping up CDs by the millions per week like they would four years later. “They should teach a class in raising kids, because it is not a thing you can prepare for,” he says. “My mom says it’s like the town from The Stepford Wives,” Fischetti admits, “but it’s a nice place to raise a family.” And he’s got a big one: a wife and five children ranging from 3 to 14. Celebration is a planned city, right next to Walt Disney World, that has “successfully combined education, health, community, technology and architecture into a new and exciting place to live, work and play,” according to its website. It’s near his home in Celebration, Florida, the town that Disney built. The Story of Huey Lewis Is Not a Tragedyįorty-five-year-old Fischetti is the musical director for his church now.
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Hale then swears his love for June, who is now the sophisticated lady she always had wanted to be, but when he refuses to avenge Buddy's death except through the law, she rebukes him. When the Falins dynamite the bridge to the mine, Buddy is accidentally killed, and June returns for the funeral. As winter descends upon the hills, Buddy learns the alphabet from Hale, but Dave, resistant to change, leaves the Tollivers. When June refuses to return home, Hale sends her to his sister's home in Louisville. After defending himself against Dave, Hale fights the Falins, while Dave escapes with June. ![]() Dave, believing Hale's business deals are a subterfuge for stealing June, goes after the couple with a rifle, but comes into trouble with the Falins, who wait for him in Gaptown. When Hale encourages June to get an education, she insists on following him into Gaptown, hoping to become sophisticated there. June visits Hale frequently with her young brother Buddy, who idolizes the engineer. Judd Tolliver refuses to sign Hale's contract when he learns the railroad will cross Falin land, but acquiesces when Hale promises financial prosperity for his indigent family. Dave plans to marry his cousin June, but she quickly becomes infatuated with the educated Hale. ![]() ![]() When Hale first arrives at the Tolliver home, he finds Dave, a visiting nephew, dying of gangrene and saves his life. As another generation upholds the vengeful "code of the Lonesome Pine," city engineer Jack Hale comes to mine the coal on their land and bring the railroad to the area. For years the Falins and Tollivers, hill families of Kentucky, have been feuding for reasons long forgotten. ![]() ![]() So now the latest incarnation is Org mode and hugo. But the itch remained… I never really liked jekyll and the ruby underneath always seemed so much black magic. I’ve never wanted a database-based blogging engine, so over the years I’ve tried PHP, nanoblogger, emacs-muse, silkpage and docbook before settling on Emacs Org mode for writing and jekyll for publishing. A hand-built journal/blog on top of that PHP, then a few experiments in moving to various static publishing systems. Long ago it started as a learning experiment with a few static HTML pages, then I added a bit of server-side includes and some very ugly PHP. On the setup window select the language and follow the instructions on screen. Run the downloaded file and allow it to run as administrator if requested. Download the latest version of the installer from gnuplot site. This example will show how to setup gnuplot in the various platforms. I try not to break URLs, so historical oddities abound. Gnuplot is a portable command-line driven graphing utility. Like the papers on my desk, or the books on the bedside table, the pile just grew… and it all grew without much plan or structure. Some of the site is organised around topics, other parts are organized by date, then there’s always the cross-references between them. Vanity site? Technology experiment? Learning tool? Blog? Journal? Diary? Photo album? I could tell you, but then I’d have to kill you… Demos for gnuplot version 5.2 (pngcairo terminal) See also the demo output for the and canvas terminals. There’s not much more I can add to who I am. Ajft looking stylish and black …The Owner ![]() But it wasn’t too difficult to cobble one together from schematics using an Arduino (I used an Arduino Nano), a 16-by-2 LCD display board, and a few discrete components. The companies listed on the MPGuino Wiki page as selling MPGuino boards or kits at one time are now out of that business. But having since sold the car in which I had installed it, I would need now to find another MPGuino for my Corolla.Īlthough the software side of MPGuino is still being actively developed, hardware for it is now harder to come by. I recall that this gizmo was very helpful. It also records the amount of fuel used and the distance traveled over longer periods, so it can display average MPG for a single trip, or for the last tank of gas. With those two signals, an MPGuino can calculate how fast the car is going and how much fuel it is using, so it can show the driver instantaneous fuel economy in miles per gallon (MPG). An MPGuino taps into two signals available at a car’s electronic control unit (ECU): One is from the vehicle-speed sensor the other runs the fuel injectors. So I decided to revisit a project I first explored in 2009, when I installed an Arduino-based fuel-economy computer dubbed “ MPGuino” in the car that I was driving at the time. ![]() But my car was manufactured years before OBD-II became standard. Owners of most vehicles can purchase, for example, a ScanGauge and plug it into the OBD-II ( On Board Diagnostics II) data port of their cars, which would allow them to view an estimate of instantaneous MPG while driving. Many newer cars show instantaneous fuel consumption on the dash. ![]() I knew, though, that it was going to be difficult to test out different driving techniques without more immediate feedback of their effects on fuel economy. And just driving it differently could also help a great deal. Possible strategies for improving its fuel economy include putting on some low-rolling-resistance tires, adding a shroud below the engine compartment (to reduce aerodynamic drag), even removing the alternator (as some racers do) or having it operate only when braking. I thought briefly about purchasing a car that got better gas mileage, but it made sense first to see how much fuel economy I could eek out from the aging econobox I am now driving: a 1991 Toyota Corolla. Russia’s invasion of Ukraine and the skyrocketing fuel prices that ensued last year got me thinking about how to cut down on my consumption of gasoline. (In Preferences, you can create a whitelist of file types you don't want ClamXav 2 to scan.) The other buttons at the top of ClamXav 2's panel are for updating its list of virus definitions, viewing a log of scans, viewing a log of updates and a Preferences button. ClamXav 2 has no prepared full-scan or quick-scan options. To begin the scan, highlight the desired folder or volume in the Source List by clicking on it, and then click the green Start Scan button at the top of the window. I dragged in the entire Macintosh HD volume. You can either click the "+" button beneath the Source List in ClamXav 2, or navigate to the target file or volume and drag it right into the Source List. For those platforms, check out our roundups of the best Mac antivirus software and the best Android antivirus apps.īefore starting a scan, you must choose which files on your hard drive you want scanned. It looks like another window of the Mac OS X's Finder files being scanned are listed in alternating blue and white rows, as in older versions of Mac OS X and iTunes.Īs free, open-source software, ClamXav doesn't come bundled with Windows or Android software the way some commercial programs do. ![]() After installation, ClamXav 2 launches and begins its first definition update.ĬlamXav 2 has the starkest interface of all the Mac antivirus programs we reviewed. You can also get a slightly different version of ClamXav 2 from the Mac App Store. ![]() Improved the default name used by the New File from Selection dialog in many contexts.Enhanced export of primer data to include options for primer length, % GC, and MW.Enabled export of all primer data if no primers are selected.Enabled drag and drop for opening files in the launch dialog, or loading files in an action dialog, or aligning sequences with the alignment list box.Modified the Import Primer from a List dialog to choose by default the last list used.Modified the Add|Import|Detect Features|Primers dialogs to remember the last column used for sorting.Added the ability to detect common features in inserted sequences.Configured the default behavior to open documents and windows on the primary display, or if other windows have been opened, on the last display that was used.Improved zooming into selected features and primers when no DNA selection is present.New “Symbols and Abbreviations” window, accessible from the Help menu.New “Analyze Selected Primer(s)” command in the Primers menu, for direction to IDT’s OligoAnalyzer website.Support for the following GenBank feature qualifiers:.Support for the following GenBank feature types:.Opening of previously generated alignments in SnapGene Viewer.Highlighting of matches for searches in both the view and the relevant scroll bar.Japanese language option for the entire interface.SnapGene can now open the following protein file formats: DNADynamo, DNASTAR (EditSeq, SeqBuilder) DNA Strider FASTA, GenBank/GenPept MacVector Vector NTI (pa4, pro, and VNTI database) ![]() Support for standalone protein sequence files, including conversion from translated DNA features to protein sequences.No Comments Changes in version 3.0 (Dec 21, 2015) New Functionality Version 3.0 By Michael | Release Notes From Blog | Ensured correct Gibson Assembly when one or more fragments are flipped.(Reported by Evan Johnson, Yelena Freyzon, and Erin Chown) Restored the normal click-and-drag selection behavior on Mac OS X 10.7.Prevented an instability issue when right clicking an aligned sequence summary in Sequence view and choosing “Hide Sequence”.Improved the flexibility of the importer for GenBank files.(Reported by Abby Sewell, Nick Matinyan, and others) Fixed an issue where aligned sequences traces sometimes failed to show chromatogram data or properly embed within the file when saving on Windows.Improved automatic annotation for misc_RNA features.Added “transOMIC” to the Sequence Author menu.Ability to use the Save menu button in the toolbar to export all primer data when no primers are selected.No Comments Changes in version 3.0.2 (Jan 2, 2016) New Functionality Version 3.0.2 By Michael | Release Notes From Blog | It shall ask for Name and Password, type in and click OK.When the dialog box opens, Right-click (ctrl+click) on the left panel and select “ Reset Printing System.” and then click OK to confirm reset.Go to Hardware Tab, then locate Print & Fax/Scan and open it.Open System Preference by clicking on Apple Icon and selecting from the menu.If you’re already done with this step, you can skip this, as it’s required to do only one time.įirst of all, we need to Reset the Printing System. Fix HP Office Jet Pro Scanning Issues After Upgrading to macOS X Yosemiteįix HP Office Jet Pro Scanning Issues After Upgrading to macOS X Yosemite Solution 1įirst of all, there are some basic steps to do, which are required before going ahead to advanced troubleshooting for fixing the driver issues of HP Office Jet Pro with Mac OS X Yosemite:. ![]() If you continue to experience problems after installing the software, use the steps below to solve additional problems. The Application will identify any missing printer software as well as any issues that may be remedied by updating to the most recent version of the software.
![]() Immediately set up a live, real-time visualization between ArchiCAD and Lumion. ![]() You will need Lumion 8.3 (or above) and ArchiCAD 21 (or above) to use the LiveSync functionality. Now, you can view and change your ArchiCAD model in real-time in Lumion, as well as export a Collada (.DAE) file from ArchiCAD and import (or re-import) it seamlessly into Lumion. The Lumion LiveSync for ArchiCAD plug-in includes two features - LiveSync and a Collada (.DAE) exporter. Model your design in its future environment. ArchiCAD in the context of the real world. Just make sure to restart ArchiCAD after doing that (if it was already running).Ĥ.2: Unzip and extract the file ' ArchiCADToLumionBridge.apx' to a folder.Ĥ.3: Follow the instructions in section 2 above.Ĥ.4: Move ' ArchiCADToLumionBridge.apx' to Installation folder\ArchiCAD (Version)\Add-Ons\ĪrchiCAD To Lumion Bridge for ArchiCAD 20ĪrchiCAD To Lumion Bridge for ArchiCAD 19ĪrchiCAD To Lumion Bridge for ArchiCAD 18ĪrchiCAD To Lumion Bridge for ArchiCAD 17ĪrchiCAD To Lumion Bridge for ArchiCAD 16 64 bitĪrchiCAD To Lumion Bridge for ArchiCAD 16 32 bitĥ. You can either re-install ArchiCAD before re-installing the Lumion plug-in, o r you can Contact Us for the APX file. If the ArchiCAD folder name or location has changed since it was originally installed, our installer won't be able to install the '.APX' files. The LiveSync installer checks which ArchiCAD versions are installed via the Windows Registry. Installation drive and folder\ArchiCAD (Version)\Add-Ons\Ĭ:\Program Files\GRAPHISOFT\ArchiCAD (Version)\Add-Ons\Ģ.4: Click on the download link above to install the plug-in.A helpful and intuitive CAD tool for construction enginnersĬombining its 3D visualization capabilities with the powerful set of modeling tools, ArchiCAD enables you to create complex models in an intuitive working environment.ĭesigned to help architects communicate their design to construction engineers, this application is capable of simulating the way a building is constructed, allowing architects to view design ideas come to life.2.2: Type ' Add or remove programs' in the Windows search field, press Enter and uninstall Lumion LiveSync for ArchiCAD (if it exists).Ģ.3: Remove all Lumion ''.APX' files from the ArchiCAD Add-Ons folder: Furthermore, is allows architects to perform dynamic building energy calculations, based on model geometry analysis. ![]() Layer management, auto-intersection, object smoothing, partial display, advanced 2D drafting and advanced shadowing and lighting options are features designed to help you create fully interactive 3D models, with details about functionality and the physical structure. Furthermore, you can create your own customized line types, vectors, symbols and images. ![]() Effortlessly create new rooms and houses from scratchĪrchiCAD enables you to manage building sections and plan rooms, interior and exterior elevations, custom materials, 3D textures, walls, stairs, columns, doors and windows with composite structures, as well as connections and solid element operations. The powerful, yet comprehensive set of tools helps you focus on the design, while the necessary documentation (dimensioning, door / window schedules, layout book, floor, foundation or beam plans) is automatically created, without compromising any detail, even for complex projects. The cloud-based BIM database provides access to a collection of thousands of GDL objects that can be included in your projects and managed using the built-in library. The application comes with direct modeling capabilities, enabling you to create custom elements with any geometry type (so-called 'MORPH' objects) by editing, shaping and moving surfaces, corners, edges and parameters without any restrictions. Shape, edit and move surfaces in a user-friendly environmentĬombining ease of use with advanced modeling capabilities, this application can fasten the designing process, from the initial concept to the final architectural sketch and documentation level. ArchiCAD provides you with a native building information modeling platform designed to help professional architects create realistic digital representations of their vision. ![]() ( 14), mis-priming can compete with amplification of the desired target sequences, thereby significantly reducing the efficiency of the amplification of the desired sequence, especially for low copy number targets. These nonspecific primer complexes may initiate the synthesis of undesired ‘mis-priming’ and ‘primer dimer’ extension products, respectively. Under these less stringent conditions, the primers, which are in a large molar excess over target, can bind nonspecifically to regions of the nucleic acid target with partial complementarity or to other primer molecules ( 14). Off-target amplifications are thought to occur during the lower temperature conditions of PCR sample preparation and thermal cycler ramping to the initial denaturation temperature. In addition, the need for improved PCR performance is imperative for the preparation of samples for cloning and next-generation sequencing applications ( 12, 13).Īlthough primer sequence and length can be carefully designed to optimize its hybridization to only the intended target sequence at the annealing temperature, PCR amplification reactions can still be plagued by off-target amplification ( 14). Robust PCR performance is of the utmost importance for high-sensitivity analytical PCR schemes, such as detection of single-copy DNA molecules ( 9), blood-borne infectious agents ( 5, 7), biohazardous microbes ( 3), defective or cancerous genes ( 4, 10, 11), single nucleotide polymorphisms ( 10, 11) and forensic samples ( 3, 8). This wide-spread technique finds utility in applications that require the amplification of a target region of interest with high specificity and accuracy ( 3–8). The ‘essential reaction components’ in a PCR cocktail include the DNA polymerase, two oligonucleotide primers, deoxynucleoside 5′-triphosphates (dNTPs), magnesium ion and other buffer components ( 1, 2). The polymerase chain reaction (PCR) is a powerful technique used to produce multiple copies of a nucleic acid region of interest. When OXP-modified primers were used as substitutes for unmodified PDE primers in PCR, significant improvement was observed in the specificity and efficiency of nucleic acid target amplification. The OXP-modified primers were tested in conventional PCR with endpoint detection, in one-step reverse transcription (RT)–PCR and in real-time PCR with SYBR Green I dye and Taqman ® probe detection. Furthermore, incubation of the OXP-modified primers at elevated temperatures was found to produce the corresponding unmodified phosphodiester (PDE) primer, which was then a suitable DNA polymerase substrate. Studies demonstrated that the presence of one or more OXP PTE modifications impaired DNA polymerase primer extension at the lower temperatures that exist prior to PCR amplification. Herein we present a novel Hot Start activation approach in PCR where primers contain one or two thermolabile, 4-oxo-1-pentyl (OXP) phosphotriester (PTE) modification groups at 3′-terminal and 3′-penultimate internucleotide linkages. Great improvements to PCR performance have been achieved by the use of Hot Start activation strategies that aim to prevent DNA polymerase extension until more stringent, higher temperatures are reached. ![]() Windows 10 allows you to link your digital license with your Microsoft account, which makes reactivating it in the future much easier (as we'll see).The polymerase chain reaction (PCR) is widely used for applications which require a high level of specificity and reliability, such as genetic testing, clinical diagnostics, blood screening, forensics and biodefense. Conversely, those who upgraded to Windows 10 from a licensed copy of Windows 7 or Windows 8.1 have a digital license and don't need a product key for activation. If you purchased your PC ready-built, it also likely came with a product key. ![]() You'll receive a product key if you buy a Windows 10 license from Microsoft or another retailer. You have two main ways to activate Windows 10: with a product key, or through a digital license. Activation checks your copy of Windows with Microsoft's servers to make sure that it's genuine. While you can download Windows 10 freely from Microsoft and install it on any computer at no cost, doing so does not activate the operating system. See our Windows 10 activation FAQ for more information. Before we proceed, let's first briefly explain what Windows activation is. ![]() A few new Ruthless-specific vendor recipes have been introduced. When an Influenced Item is reforged or the Influenced Mod is removed, its influence is lost. Veiled Chaos Orbs, Orbs of Binding, Orbs of Dominance, Influenced Exalted Orbs and Awakener's Orbs cannot be obtained. ![]() ![]() The crafting bench cannot be accessed and recipe plinths cannot be found. The rate at which you receive shards of crafting currency when vendoring items has been decreased. The drop rate of crafting currency items has been massively decreased. Crafting is a powerful luxury that you'll have to use sparingly for the right items. It's hard to find magic, rare and unique items, and it's even harder to craft them. Limited Crafting In Ruthless, most of your items come from killing monsters. Many bosses like Atziri that guarantee unique drops still do, but are often harder to access. Many deterministic items like Divination Cards or Oni-Goroshi cannot be obtained. In Ruthless, getting items from the core drop pool is a valuable reward, especially when there is an item rarity bonus. Most league reward systems have been changed to only grant item rewards that are specific to that league (for example, Cluster Jewels only come from Delirium), plus items from the core drop pool. Rings, Amulets and Belts are much rarer than in regular Path of Exile. The rate of finding magic, rare and unique items has been massively reduced throughout all game content. In Ruthless, the quantity of items dropped has been massively reduced throughout all game content. Every item that drops has the potential to be an upgrade to your current gear. Item Scarcity The core Ruthless experience is that you don't have strong enough items to handle the content. Aspects like item scarcity and support gems being valuable really feel like the early days of Path of Exile, just without desync. We picked the name partly because it was what the third of four difficulty levels was called back in closed beta. But that orb lets you convert any base type of your choice to a rare item. You might only find one Orb of Alchemy throughout the whole campaign. Ruthless is a mode where you find very little crafting currency. But finding a rare item of a base type you're looking for feels amazing. You don't see a lot of magic items, and even fewer rare items. Ruthless is a mode where most items are normal rarity. But each ring you find represents a huge power boost. You might get to act four without equipping a pair of rings. Ruthless is a mode where you barely find any items. High level characters and good rare items infer immense bragging rights. Traditionally weak items are suddenly very valuable. Even reaching higher campaign acts, let alone maps, is an achievement. It redefines the entire game as the endgame. Ruthless is a mode that re-imagines traditional understanding of where Path of Exile's endgame is. In a world where your items are far below par, every item drop has the potential to be the breakthrough one you need. It's quite difficult, but overcoming that difficulty feels highly rewarding. Ruthless is a mode about friction, tension and anticipation. It's a pet project that some senior designers have been working on in their spare time over the last 18 months. Ruthless is not consuming significant development resources. You can play it for free and your existing microtransactions will work in it. ![]() Ruthless is not monetised any differently. In fact, if we felt a particular change was good for the regular game, we'd just make it there to benefit everyone. A change being made in Ruthless is not an indication that we will make that change in the regular game. It's a challenging mode for a specific type of player. Ruthless is not a replacement for regular Path of Exile. Some players really enjoy the additional constraints as a way of enhancing their game experience. If you don't like the sound of it, then it's probably best you continue playing the regular Path of Exile modes. There are a lot of other small changes that aren't covered here, but this article should give you a decent overall impression of Ruthless. Only significant changes are described, but without numerical detail. This article is not a set of patch notes. Ruthless (previously codenamed "Hard Mode") is an additional character creation flag alongside Hardcore and Solo Self-Found that allows you to opt-in to extreme item scarcity and various other changes. |
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